Table 1.
Prohead protease processed segments of head proteins from the φKZ tailless mutant determined by mass spectrometry. Proteins or segments predicted to be present in the mature virion in more than 100 copies are bolded.
| Residue2 | ||||||
|---|---|---|---|---|---|---|
| gp1 | Slice | Start | Stop | Mw3 | SC4 | SC/Mw5 |
| 84 | 10 | 11* | 413(C) | 45.7 | 34 | 0.7 |
| 86 | 8 | 123* | 428(C) | 35.4 | 47 | 1.3 |
| 89N | 1 | 1 | 122* | 13.1 | 18 | 1.4 |
| 89C | 5 | 156* | 387(C) | 26.1 | 117 | 4.5 |
| 92 | 10 | 86* | 440(C) | 41.1 | 11 | 0.3 |
| 93 | 7 | 157* | 435(C) | 30.9 | 177 | 5.7 |
| 94N | 1 | 12* | 95* | 9.2 | 11 | 1.2 |
| 94M | 1 | 96* | 275* | 20.1 | 15 | 0.7 |
| 94C | 5 | 276* | 505(C) | 26.4 | 14 | 0.5 |
| 95 | 7 | 261* | 547(C) | 31.6 | 177 | 5.6 |
| 96 | 8 | 66* | 371(C) | 34.9 | 6 | 0.2 |
| 97N | 14* | 66* | 5.8 | 24 | 4.1 | |
| 97C | 7 | 533* | 816(C) | 30.7 | 152 | 5.0 |
| 120, capsid | 12 | 164* | 747(C) | 64.9 | 1672 | 25.8 |
| 129N | 3 | 1 | 262* | 29.3 | 5 | 0.2 |
| 129C, portal | 12 | 263* | 896(C) | 70.1 | 89 | 1.3 |
| 153 | 5 | 53* | 304(C) | 28.2 | 42 | 1.5 |
| 162N | 3/4 | 18* | 137* | 13.2 | 41 | 3.1 |
| 162C | 6 | 259* | 522(C) | 29.0 | 156 | 5.4 |
| 163 | 8 | 55* | 395(C) | 38.1 | 45 | 1.2 |
| 175 | 4 | 1 | 210* | 23.7 | 39 | 1.6 |
| 177N | 1 | 1 | 60* | 6.4 | 9 | 1.4 |
| 177C | 11 | 61* | 519(C) | 52.4 | 126 | 2.4 |
| 180_180_2 | 12 | 1 | 505 | 57.8 | 36 | 0.6 |
| 184N | 3 | 1 | 108* | 12.1 | 6 | 0.5 |
| 184C | 1 | 109* | 230 | 13.2 | 21 | 1.6 |
| 203 | 12 | 1 | 653* | 75.3 | 71 | 0.9 |
| 303N | 8 | 61 | 268 | 22.8 | 38 | 1.7 |
| 303M | 1 | 269 | 366(C) | 11.1 | 17 | 1.5 |
| 303C | 7 | 367 | 646(C) | 31.3 | 62 | 2.0 |
The gene product name is appended with N (for N-terminal), M (for middle), or C (for C-terminal) when more than one segment of a gene product were detected in the virion.
A coordinate is appended with an * if confirmed by a semi-tryptic spectrum, or by (C) for an end defined by the stop codon. Semi-tryptic spectrum assignments were above 95% confidence except as noted in Supplementary Table 2.
The molecular weight (MW) was calculated from the sequence.
Peptide spectral counts (SC) were summed over all slices.
SC/MW values were calculated as an approximate indicator of abundance of the polypeptides per virion.