Figure 8. ΔopuA-D strains require the otsBA locus to out-compete wild-type strains in mice.
Mice were orally infected with a 1:1 mixture of the strains indicated on the x-axes. After three weeks, mice were euthanized and their organs (cecum (C), Peyer’s patches (PP), mesenteric lymph nodes (MLN), spleen (S), and liver (L)) immediately collected for analysis. Bacteria were enumerated after plating homogenized A) cecum, B) Peyer’s patches, C) mesenteric lymph nodes, D) spleen, and E) liver. Each symbol represents one mouse, N = 6–23. P values are # < 0.01 compared to the null hypothesis and * < 0.05 compared to ΔopuA-D vs WT.