Skip to main content
. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Mol Microbiol. 2012 Mar 9;84(2):296–309. doi: 10.1111/j.1365-2958.2012.08022.x

Figure 9. Fasting time affects gastric pH and colonization of ΔopuA-D strains in mice.

Figure 9

A) Mice were fasted for 9–12 hours (closed circles) or 2–4 hours (open circle) prior to oral inoculation with a 1:1 mixture of the ΔopuA-D and wild-type strains. After three weeks, mice were euthanized and their organs (cecum (C), Peyer’s patches (PP), mesenteric lymph nodes (MLN), spleen (S), and liver (L)) immediately collected for analysis. Each symbol represents one mouse, N=13–23. Asterisks indicate P < 0.01 (*), 0.001 (**) or 0.001 (***) compared to the null hypothesis. B) The pH of the gastric content was measured for mice fasted for three hours (short) and 11 hours (long); control (ctrl) mice were not fasted. Error bars are SD, N= 3–4 mice. Asterisks indicate P < 0.05 (*) and 0.01 (**) compared with control.