Table 1.
Strain | Invasion of T84 cells (% inoculum)a |
---|---|
Wild-type | 2.23 ± 0.28 |
Δ σ 28 | 0.16 ± 0.06* |
Δ 0996 | 0.20 ± 0.05* |
Δ fspA1 | 2.59 ± 0.15 |
Δ fedA | 0.22 ± 0.05* |
Δ fedB | 1.80 ± 0.34 |
Δ fedC | 2.17 ± 0.38 |
Δ fedD | 1.61 ± 0.30 |
Δ ciaI | 1.11 ± 0.35* |
Percent invasion was determined by comparing the number of intracellular bacteria surviving a 2 h gentamicin treatment of infected T84 cells compared to the number of bacteria in the infecting inoculum (approximately 3.0 × 106 cfu). Each assay was performed in triplicate, and at least three biological replicates were performed. The average percent invasion +/- standard error for each strain is presented. Statistically-significant differences in invasion between wild-type C. jejuni and mutant strains are indicated
P-value < 0.05.