Table 2.
Invasion capacity of wild-type C. jejuni 81-176 SmR and ciaI mutant strains for T84 colonic cells.
| Strain | Invasion of T84 cells (% inoculum)a |
|---|---|
| Wild-type | 2.08 ± 0.22 |
| Δ ciaI | 1.28 ± 0.13* |
| ciaI K42A | 1.44 ± 0.27 |
| ciaI LL153-154AA | 1.74 ± 0.18 |
a
Percent invasion was determined by comparing the number of intracellular bacteria surviving a 2 h gentamicin treatment of infected T84 cells compared to the number of bacteria in the infecting inoculum (approximately 3.0 × 106 cfu). Each assay was performed in triplicate, and at least three biological replicates were performed. The average percent invasion +/- standard error for each strain is presented. Statistically-significant differences in invasion between wild-type C. jejuni and mutant strains are indicated
*
P-value < 0.05.