Fig 1.
Phenotyping of EPCs. Mononuclear cells were cultured on fibronectin-coated plates in EGM2 for 4 days. A: Phase contrast image of day 4 EPCs (× 20 magnification). B–E: FACS analysis of cell surface markers on EPCs. Shown are representative data from at least 3 independent experiments for each marker. The open black-lined histograms represent tested antibodies, and filled histograms represent the control IgG. F: Human EPCs expressed receptors that bind to BDNF, TrkB and p75NTR. Positive controls (Post) for p75NTR and TrkB are SK-N-MC cell lysate and mouse brain, respectively. G: mRNA expressions of TrkB and p75NTR in day 4 EPCs and circulating mononuclear cells (MNC).
