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. Author manuscript; available in PMC: 2013 May 1.

Published in final edited form as: Microvasc Res. 2012 Jan 9;83(3):366–371. doi: 10.1016/j.mvr.2012.01.001

BDNF protected EPCs from oxidative stress. A and B: EPCs were incubated with indicated treatments, then were subjected to TUNEL assay (A, n=5–8, *P<0.05, compared to other 2 groups), or Western blotting (B, n=5, *P<0.05, compared to other two groups, **P<0.05, compared to control). C: MNC were seed in 6-well plates (20×10⁶ cells/well, in duplicates) and cultured in EGM2 for 4 days. The non-adherent cells were washed away, the attached cells (EPCs) were treated with EGM2 (control), EBM2, or EBM2 +BDNF for 48 h (treatment was refreshed every 24 h). The attached cells were collected by trypsinization and cell numbers were counted using a hemocytometer. Data are presented as % to control (EGM2 alone); n=7, *P<0.05.

BDNF protected EPCs from oxidative stress. A and B: EPCs were incubated with indicated treatments, then were subjected to TUNEL assay (A, n=5–8, *P<0.05, compared to other 2 groups), or Western blotting (B, n=5, *P<0.05, compared to other two groups, **P<0.05, compared to control). C: MNC were seed in 6-well plates (20×10⁶ cells/well, in duplicates) and cultured in EGM2 for 4 days. The non-adherent cells were washed away, the attached cells (EPCs) were treated with EGM2 (control), EBM2, or EBM2 +BDNF for 48 h (treatment was refreshed every 24 h). The attached cells were collected by trypsinization and cell numbers were counted using a hemocytometer. Data are presented as % to control (EGM2 alone); n=7, *P<0.05.