Table 2.

Levels of catalytic subunits of s-20S and i-20S in EAE (pmol/mg protein)

	Control young	Acute EAE	Control old	Chronic EAE
β1	1.73 ± 0.20	1.99 ± 0.11	1.70 ± 0.14	1.17 ± 0.05*
β2	0.95 ± 0.10	0.78 ± 0.16	0.49 ± 0.05	0.59 ± 0.30
β5	1.57 ± 0.10	1.55 ± 0.06	1.25 ± 0.24	0.41 ± 0.10*
iβ1	0.99 ± 0.30	2.37 ± 0.20*	0.89 ± 0.09	0.72 ± 0.13
iβ2	2.03 ± 0.49	3.59 ± 0.50*	1.45 ± 0.08	1.04 ± 0.22
iβ5	0.92 ± 0.06	1.69 ± 0.26*	0.86 ± 0.07	1.02 ± 0.23

The concentration of each standard and immuno catalytic subunit in control and EAE cerebella was determined by western blot analysis (Fig. 1 and 2) using calibration curves made with increasing amounts of either purified standard proteasome or immunoproteasome. Values are the mean ± SEM of 3 animals per experimental group. Asterisks denote values that are significantly different (p<0.05) from control.