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A drop of pH from 7.2 to 6.1 decreases the affinity of FluoZin-3 and Fura-2FF for Zn2+. (a) FluoZin-3 F488 signal was monitored in vitro at different [Zn2+] at a pH of 7.2 and 6.1. The data were fitted as described in the Methods and yielded the indicated apparent Kd values. (b) Analogous data obtained for the Fura-2FF F346 signal.
