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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Mol Microbiol. 2012 Mar 15;84(2):203–224. doi: 10.1111/j.1365-2958.2012.08023.x

Fig. 3. All branches have inert PG at their poles.

Fig. 3

E. coli CS703-1, a mutant lacking PBPs 1a, 4, 5, 6, 7, AmpC and AmpH, was grown in LB containing 100 µg/ml of D-cys for three generations, after which cells were chased in the absence of D-cys by growing them in LB plus 1 µg/ml of aztreonam (to inhibit cell division) for 2 h (~3 mass doublings). After the chase, sacculi were isolated and immunolabelled to detect D-cys residues, as described (de Pedro et al., 1997). This figure is a mosaic of images taken from different fields of view. The scale bar equals 5 µM.