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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Mol Microbiol. 2012 Mar 15;84(2):203–224. doi: 10.1111/j.1365-2958.2012.08023.x

Fig. 5. LMW PBP mutants have aberrant Z rings.

Fig. 5

E. coli strains LP18-1 (wild type) (A, B), LP1 (ΔPBPs 4, 5 and 7) (C, D) and LP17-1 (ΔPBPs 4, 5, 6, 7, ampC and ampH) (E, F) were grown and imaged as described in Experimental Procedures. Cells in panels B, D and F were incubated for ~1 mass doubling in the presence of 1 µg/ml of aztreonam. FtsZ polymers that appear to be spirals or helices, incomplete Z rings, arcs and FtsZ polymers that did not form compact rings were considered to be spirals, and are illustrated by the Z ring marked by asterisks (*). Rings that are broader (take up more space than normal along the long axis of the cell) are represented by a triangle (▲); these Z-rings may be slanted but appear as rings instead of bands, probably because they are oriented differently. Slanted rings that are not perpendicular to the long axis of the cell are illustrated by those marked with white arrows. All images have the same magnification; the scale bar in panel A equals 5 µm.