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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Mol Microbiol. 2012 Mar 15;84(2):203–224. doi: 10.1111/j.1365-2958.2012.08023.x

Fig. 7. sfGFP-PBP6 localizes to the septum in cells lacking PBP5.

Fig. 7

E. coli CS17-1 (ΔPBP6) expressing DsbA-SS-sfGFP-PBP6 from pLP602 (A and B), or DsbA-SS-sfGFP-PBP6S66A from pLP607 (C and D). E. coli CS12-7 (Δ PBP5) expressing DsbA-SS-sfGFP-PBP6 from pLP602 (E and F), or DsbA-SS-sfGFP-PBP6S66A from pLP607 (G and H). Cells were grown and imaged as described in Experimental Procedures. Cells in panels B, D, F and H were filamented by treating with 1 µg/ml of aztreonam for 1 mass doubling. Each panel has a phase contrast image on the left and the corresponding fluorescence image on the right. All images have same magnification; the scale bars in panels A and E equal 5 µm.