Table 1.
FtsZ overexpression does not change peptidoglycan composition.
| Molecular percentage | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PG Composition |
Wild Type | Δ 4, 7 | Δ 5 | Δ 4, 5, 7 | ||||||||
| - | FtsZ, A, Q | FtsZ | - | FtsZ, A, Q | FtsZ | - | FtsZ, A, Q | FtsZ | - | FtsZ, A, Q | FtsZ | |
| Pentapetides | 1.5 | 1.1 | 1.0 | 0.7 | 1.4 | 1.1 | 7.4 | 7.2 | 6.9 | 21.7 | 25 | 23.6 |
| Cross linkage | 30.9 | 30.1 | 30.0 | 39.4 | 37.7 | 38.6 | 31.8 | 30.4 | 31.8 | 38.7 | 39.1 | 39.6 |
| Monomers | 69.0 | 69.6 | 69.6 | 61.0 | 62.3 | 61.5 | 67.0 | 68.4 | 66.8 | 62.0 | 61.9 | 62.2 |
| Dimers | 26.3 | 26.0 | 26.4 | 36.0 | 34.3 | 35.4 | 29.1 | 27.9 | 28.6 | 34.9 | 34.8 | 33.9 |
| Trimers | 2.3 | 2.1 | 1.8 | 1.7 | 1.7 | 1.6 | 1.3 | 1.3 | 1.6 | 1.9 | 2.1 | 2.8 |
| Lipoprotein | 11.4 | 9.3 | 9.5 | 9.8 | 10.2 | 9.5 | 9.3 | 8.5 | 10.4 | 7.5 | 7.2 | 7.0 |
| Anhydro | 1.9 | 1.7 | 1.5 | 1.0 | 2.1 | 1.6 | 1.7 | 0.9 | 2.9 | 1.7 | 1.9 | 0.8 |
| DAP-DAP | 3.2 | 2.5 | 2.6 | 4.0 | 3.5 | 2.8 | 3.2 | 2.7 | 3.3 | 1.8 | 2.3 | 2.0 |
Peptidoglycan was isolated from E. coli strains harboring pLP322 (−), pZAQ (FtsZAQ) or pLPZ (FtsZ), and the murein composition was analyzed by HPLC as described in Experimental Procedures. The molecular percentage of each muropeptide is reported. Strains that did not harbor any plasmid showed similar PG compositions (not shown). The results represent the average PG composition derived from two independent experiments.