Table 2.

The frequency of abnormal FtsZ rings increases with the loss of multiple PBPs.

Percentage of FtsZ polymers
Strain	PBPs Deleted	Normal	Spirals	No Rings	Broad Rings	Slanted Rings	Total Abnormal Z-polymers	No. of Cells counted
LP18-1	-	87	-	13	-	-	-	554
LP1	4, 5 & 7	65.5	11	15.8	2.5	5.2	18.7	557
LP16-1	4, 5, 6 & 7	62.5	14.4	13.2	5.3	4.6	24.3	560
LP17-1	4, 5, 6, 7, AmpC & AmpH	39.7	28	18.4	3.4	10.5	42.0	554

FtsZ-GFP was visualized as described in Experimental Procedures. The percentages represent cells with one perpendicular FtsZ ring (Normal), cells that had no rings or spirals (No-rings), and cells with other forms of FtsZ (e.g., spiral, helix, incomplete Z-rings, arcs and polymers not forming compact rings) (Spirals). Representatives of spiral forms are marked with asterisks (*) in Fig. 5. In addition, other cells were classified as having FtsZ rings that were extended or tilted in a non-native orientation (Wide Rings), and representatives of this type are marked by triangles (▲) in Fig. 5. Finally, some cells had FtsZ rings oriented at an angle other than 90° to the cell axis (Slanted Rings), and representatives are marked with white arrows in Fig. 5.