Table 3.
PBP6 and DacD active site mutants do not have D, D-CPase activity.
| Molecular Percentage | |||||
|---|---|---|---|---|---|
| PG Composition |
- | PBP6 | PBP6S66A | DacD | DacDS63A |
| pLP18-Kan | pLPKC604 | pLP606 | pLPKC653 | pLP655 | |
| Monomers | 57 | 56.7 | 55.8 | 58.5 | 60.9 |
| Dimers | 39.5 | 39.8 | 40.5 | 39.0 | 36.2 |
| Trimers | 3.5 | 3.6 | 3.7 | 2.5 | 2.9 |
| Lipoprotein | 2.6 | 3.6 | 2.6 | 8.4 | 3.8 |
| Anhydro | 4.8 | 5.0 | 5.3 | 3.8 | 4.4 |
| DAP-DAP | 0.31 | 0.2 | 0.3 | 2.8 | 0.3 |
| Pentapeptide | 75.1 | 66.6 | 75.8 | 3.1 | 70.1 |
| Penta-glycine | 5.8 | 6.2 | 6.8 | 0.3 | 7.3 |
| Cross-Linkage | 46.5 | 47.0 | 48.0 | 44.0 | 42.0 |
| Average Length | 20.8 | 20.1 | 19.0 | 26.1 | 22.5 |
Peptidoglycan was isolated from E. coli CS704-1 harboring pLP18-Kan, pLPKC604, pLP606, pLPKC653 or pLP655. The murein composition of each sample was analyzed by HPLC as described in Experimental Procedures. The molecular percentage of each muropeptide is reported.