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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Neurobiol Dis. 2012 Feb 4;46(2):325–335. doi: 10.1016/j.nbd.2012.01.013

Figure 5. Increased activation of PP1 and PP2A in the Twitcher sciatic nerve.

Figure 5

A, B) Quantitation of PP1 (A) and PP2A (B) enzymatic activity in lysates from P7 and P30 sciatic nerves of WT and Twitcher mice showed that both enzymes in Twitcher nerves were strongly over-activated at P30, while only PP2A was significantly more active in the P7 tissue. Activity is expressed as micromol substrate (DiFMUP)/mg protein/min. Data are mean ± SEM of three replicates. Data were analyzed by ANOVA/post hoc paired test. Asterisk indicates p<0.01. C–F) Representative immunohistochemical staining for PP2A in P30 Twitcher (C,D) and WT (E,F) sciatic nerves shows the higher frequency of fibers intensely stained for PP2A in the Twitcher nerve (black arrows in C and D). Rarely, PP2A+ myelinating Schwann cells were also detected in Twitcher tissue (asterisks in D). Enlarged in D, the presence of PP2A in a mutant axon is readily observed (black arrow in D), surrounded by the myelinating sheath (white arrow in D).