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. Author manuscript; available in PMC: 2013 Apr 27.
Published in final edited form as: Transplantation. 2012 Apr 27;93(8):813–821. doi: 10.1097/TP.0b013e318247a717

Figure 3. T cell proliferation and IFNγ and peforin/granzyme B expression in one-way CFSE-MLR.

Figure 3

Analyses were performed after excluding PKH-26+ stimulator cells and gating on CD3+CD8− (CD4+) and CD3+CD8+ T cells at the end of the 5-day MLR. (A) Proliferation of responder CD4+ and CD8+ T cells, presented as proportion (%) of cells that diluted CFSE, after stimulation with allogeneic donor (R+D) or third party PBMC (R+3rd). (B) IFNγ production by proliferated CD4+ and CD8+ T cells 4 hrs after re-stimulation with donor or third party CD3-depleted allogeneic PBMC. (C) Perforin/granzyme B expression by proliferated responder CD4+ and CD8+ T cells. HC (n=14), quiescent (n=12), reactive (n=4), and ACR (n=2). *p<0.05; ** p<0.01; ***p<0.005.