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Sedimentation velocity analysis of the Hsp27 Δ1−24 N-terminal deletion variant (Tris−HCl buffer (20 mM (pH 8.4), 100 mM NaCl)). c(s) distribution: (light gray) 0.4 mg/mL, 40 °C; (dark gray), 0.65 mg/mL, 40 °C; (black), 1.58 mg/mL, 40 °C, (- − -) 1.23 mg/mL, 20 °C. Inset, SEDPHAT analysis for a mixture of dimer, tetramer, hexamer and octamers.
