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. 2011 Nov 4;21(1):122–133. doi: 10.1002/pro.761

Figure 3.

Figure 3

Sedimentation velocity analysis of wild-type Hsp27 (gray) and the Δ182–205 variant (black). Top panel: c(s) distribution of wild-type Hsp27 in Tris−HCl buffer (20 mM (pH 8.4), 100 mM NaCl); (···) 20 °C (0.50 mg/mL, 22 μM), (——), 40 °C (0.56 mg/mL, 25 μM). Center panel: c(s) distribution of Δ182–205 variant in Tris−HCl buffer (20 mM (pH 8.4), 100 mM NaCl)), (···) 20 °C (0.38 mg/mL, 19 μM). (——), 40 °C (0.33 mg/mL, 16 μM). Bottom panel: c(M) distribution of the Δ182–205 variant at 40 °C.