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Chaperone activity of Hsp27 variants (sodium phosphate buffer (pH 6.7 (20 mM) 100 mM NaCl) at (A) 20 °C and (B) 40 °C: (——), DTT-induced aggregation of insulin (40 μM) alone; (•), Δ1–14/insulin (mol/mol), 0.02:1; (○), Δ1–14/insulin (mol/mol), 0.1:1; (▪) Δ182–205/insulin (mol/mol), 0.02:1; (□), Δ182–205/insulin (mol/mol), 0.1:1.
