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Percentage of Inhibition of WT and Variant Forms of Hsp27
| Protein | T (°C) | % inhibition | Insulin:Hsp |
|---|---|---|---|
| Wild-type Hsp27 | 20 | 9.3 | 1:0.05 |
| 40 | 48.4 | ||
| Δ1–14 variant | 20 | 1 | 1:0.1 |
| 40 | 7.8 | ||
| Δ182–205 variant | 20 | −43 | 1:0.1 |
| 40 | 39 |
Inhibition was calculated as ((Iins − Iins + Hsp27)/Iins) × 100) where I is the intensity of light scattering at 30 min (20 °C) or 20 min (40 °C).
