Table III.
Summary of Parameters Derived from Single- and Bi-exponential Decay Fits to Data Obtained for the FRET Experiments with Wild-type and Δ1–14 Hsp27
| Wild-type Hsp27 | Δ1–14 variant | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
 |
 |
 + A2e
|
|||||||||
| T (°C) | tmrt (min) | A | R2 | tmrt (min) | A | R2 | tmrt1 (min) | A1 | tmrt2 (min) | A2 | R2 |
| 20 | 22 ± 3 | 0.39 ± 0.01 | 0.983 | 26 ± 5 | 0.105 ± 0.007 | 0.941 | 15 ± 7 | 0.06 ± 0.02 | N/A | N/A | 0.957 |
| 30 | 5.8 ± 0.9 | 0.39 ± 0.01 | 0.985 | 18 ± 2 | 0.160 ± 0.006 | 0.981 | 13 ± 5 | 0.14 ± 0.04 | N/A | N/A | 0.986 |
| 37 | 3.3 ± 0.5 | 0.45 ± 0.04 | 0.964 | 31 ± 6 | 0.20 ± 0.01 | 0.945 | 4 ± 1 | 0.12 ± 0.02 | 70 ± 20 | 0.18 ± 0.02 | 0.982 |
| 40 | 1.2 ± 0.1 | 0.38 ± 0.02 | 0.984 | 26 ± 3 | 0.26 ± 0.01 | 0.919 | 1.4 ± 0.8 | 0.13 ± 0.02 | 37 ± 4 | 0.25 ± 0.01 | 0.958 |