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Geminin and Emi1 depletion in pluripotent cells results in induction of DNA damage proteins. Geminin and Emi1 depletion in P19 EC cells induces upregulation of γ-H2AX, Rad51 and replication protein A (RPA) foci (green) relative to unirradiated controls at 48 h post-transfection. Irradiated positive controls were treated with 2 Gy. Total DNA, red. Scale bar = 20 µm.
