Figure 3.

PAX3-FKHR-dependent gene transcription is downregulated in ARMS cells grown in differentiation-permissible conditions. (A) Luciferase activity in ARMS-derivative, PAX3-FKHR-responsive 6XPRS-Luc reporter cells grown in GM or DM for 2 d. Values are normalized to amount of protein in lysates. (B) Levels of GFP in ARMS-325-6XPRS-GFP reporter cells grown as in (A). (C) Quantitative real-time PCR analysis of MyoD in ARMS-T and ARMS-325 cells cultured as in (A). (D) RT-PCR analysis of PAX3-FKHR and β-actin in the indicated ARMS cells grown as in (A). (E) Immunoblot analysis of extracts of indicated ARMS cells grown as in (A), probed with FoxO1/FKHR and β-actin antibodies. For all applicable parts, error bars are ± SEM (n = 3).