Figure 5.

AKT hyperactivation associates with PAX3-FKHR phosphorylation in ARMS cells under differentiation-permissible conditions. (A) Immunoblot analysis of extracts of indicated ARMS cells cultured in GM or DM for 2 d, probed with indicated antibodies. (B) Immunoblot of extracts of indicated ARMS cells cultured in GM or DM with or without LY for 2 d, probed with FoxO1/FKHR or β-actin antibodies (upper). Relative level of phosphorylated PAX3-FKHR (upper) is presented as a bar graph (lower). (C) Immunoblot analysis of cell extracts used in (B), probed with indicated antibodies.