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. 2012 Mar 1;11(5):895–908. doi: 10.4161/cc.11.5.19346

Figure 8.

Figure 8

Ectopic MyoD expression rescues transcriptional inactive PAX3-FKHR inability to induce terminal differentiation of ARMS cells. (A) Luciferase activity in ARMS-325-6XPRS-Luc cells grown in GM or DM for the indicated times in days. Values are expressed after protein normalization. Error bars, ± SEM (n = 3). (B) Immunoblot of extracts of ARMS-325 cells cultured as in (A), probed with indicated antibodies. (C) Immunoblot of extracts of ARMS-325 and its derivative MyoD overexpressed ARMS-MyoD cells grown in GM, probed with indicated antibodies. (D) Immunoblot of extracts of ARMS-MyoD cells cultured as in (A), probed with indicated antibodies. (E) Immunofluorescence of muscle MyHC of ARMS-MyoD cells grown in GM or DM for 10 d.