Figure 7.
Liver regeneration after PH causes a rapid induction of CCND1 expression and miR-33 downregulation. (A) Time-course analysis of Ki67-positive cells from liver sections of mice subjected to PH. The computed assisted quantification of the proliferating cells (red) vs. the total number of cells (blue) is shown at the graph. (B) Western blot analysis of PCNA and cleaved caspase 3 expression from liver lysates of mice subjected to PH. (C) Time-course mRNA expression analysis of miR-33, SREBP-2, CDK6 and CCND1 from liver samples of mice subjected to PH. Data are the mean ± SEM (n = 6 mice).