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Shelterin proteins physically interact with BER proteins. Lane 1 indicates a molecular weight marker. Lane 2 contains IgG alone. Lane 3 contains each BER protein probed by western blot analysis. Lanes 4–6 contain immunoprecipitates of either POT1, TRF1 or TRF2, respectively, that were probed by western blot analysis with antibodies against the BER proteins. The specific procedure is included in the Materials and Methods section.
