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. 2012 Apr 1;26(7):657–667. doi: 10.1101/gad.186858.112

Figure 1.

Figure 1.

Loss of both Rev-erb subtypes renders MEFs arrhythmic. Gene expression over a 40-h period in synchronized wild-type (WT) or Rev-erbα knockout (αKO) MEFs transduced with adenovirus encoding a shRNA targeting Rev-erbβ (Revβ) or βgal as control. The experiment was performed in triplicate, and SEM is indicated by vertical bars. All values have been normalized to Arbp mRNA expression and the 8-h time point in the wild-type control. (A) Rev-erbα mRNA levels in wild-type MEFs infected with control virus (WT/shβgal) or Rev-erbβ knockdown virus (WT/shRevβ) and Rev-erbα-null MEFs infected with control virus (αKO/shβgal) or Rev-erbβ knockdown virus (αKO/shRevβ). (B) Rev-erbβ mRNA levels in the cells described in A. (C) Bmal1 mRNA levels in WT/shRevβ and αKO/shβgal cells. (D) Bmal1 mRNA levels in WT/shβgal and αKO/shRevβ cells. (E) Cry1 mRNA levels in WT/shRevβ and αKO/shβgal cells. (F) Cry1 mRNA levels in WT/shβgal and αKO/shRevβ cells.