Figure 5.

Stg or Cdk1^AF mRNA, coinjected with CycB mRNA, accelerate replication, while dsRNA against mitotic cyclins slows replication. Stills from Supplemental Movie S6. Embryos were filmed after injection with purified GFP-PCNA protein and nothing (A,F), Stg and CycB mRNAs (B), CycB mRNA (C), Cdk1^AF and CycB mRNAs (D), Cdk2^AF and CycE mRNAs (E), or dsRNA against CycA, CycB, and CycB3 (G). Time into interphase 14 is marked above the micrographs. An asterisk (*) before the time indicates a micrograph in which no PCNA foci are visible. In some panels, an open arrowhead highlights the last visible late PCNA focus. Also, in some later time-point micrographs (e.g., A, 44:07), the nuclei can be observed changing shape, which is a normal consequence of cellularization that is happening during cycle 14. Bar, 10 μm.