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. 2012 Mar;14(3):178–189. doi: 10.1593/neo.111444

Figure 3.

Figure 3

Effect of siRNA-mediated suppression of IKK1 and/or IKK2. (A) LNCaP cells were transfected with the indicated IKK siRNA. Four days after transfection, viable cell numbers were determined and plotted as mean ± SD. (B) Efficiency of knockdown corresponding to A was checked by immunoblot analysis. The same membrane was reprobed with antibodies recognizing AR and ERK2. (C) LNCaP cells were transfected with IKK1 and/or IKK2 siRNA, as indicated. After 72 hours, cells either were left untreated (-TNFα) or were stimulated with 10 ng/ml TNFα for 20 minutes. EMSA for NF-κB or Oct probes was performed with nuclear extract of these cells (upper panel). Corresponding cytoplasmic extracts were used for immunoblot analysis with indicated antibodies (lower panel).