Figure 3.

NTN4 expression is downregulated in glioblastoma specimens and associates with reduced patient survival. (A) On the basis of 425 primary GBM tumors from TCGA [27,33], bioinformatics analysis revealed that the expression of NTN4 was reduced to 33% in grade 4 glioblastoma, compared with normal brain tissue. (B) Kaplan-Meier survival analysis showed that the low expression of NTN4 predicts poor patient survival. Black line represents all glioblastoma samples with low expression of NTN4 compared with normal tissue (fold change < 0.5), and gray line represents a group of glioblastoma samples which have similar expression of NTN4 to the normal brain tissue. Dashed lines represent 0.95 confidence intervals for the curves with the corresponding color. P < .043. (C) The expression of ITGB4 increased 2.9-fold in grade 4 glioblastoma, compared with normal brain tissue. (D) Kaplan-Meier survival analysis showed that the upregulated expression of ITGB4 predicts poor patient survival. Gray line represents all glioblastoma samples with high expression of ITGB4 compared with normal tissue (fold change > 3), and black line represents a group of glioblastoma samples that have similar expression of ITGB4 to the normal brain tissue. P < .029. (E) The expression of UNC5B does not significantly change in glioblastoma compared with the normal brain tissue.