Appendix Table A1.

Cross-Validation of Support Vector Machine Classification on Different Treatment Types

Unsynchronized
	Classifier output
True class	Ana	Cyto	Inter	Meta	Multi-nuc	Noncell	Prom	Pro	Telo	#	TPR	PR
Anaphase	6	1	0	1	0	0	1	0	0	9	0.67	0.55
Cytokinesis	1	11	0	0	0	0	0	0	2	14	0.79	0.35
Interphase	0	3	649	0	103	86	0	8	25	874	0.74	0.97
Metaphase	3	1	0	9	0	0	16	2	0	31	0.29	0.60
Multi-Nuclear	0	0	10	0	23	1	0	1	0	35	0.66	0.18
Noncell	0	1	3	0	0	12	0	0	1	17	0.71	0.11
Prometaphase	1	0	0	4	0	0	7	0	0	12	0.58	0.29
Prophase	0	0	0	1	0	0	0	5	1	7	0.71	0.28
Telophase	0	14	5	0	0	3	0	2	12	36	0.33	0.29

Single thymidine block and release
	Classifier output
True class	Ana	Cyto	Frag	Inter	Meta	Multi-nuc	Noncell	Prom	Pro	Telo	#	TPR	PR
Anaphase	6	1	0	0	3	0	0	5	0	0	15	0.40	0.21
Cytokinesis	2	41	0	0	1	0	0	1	3	6	54	0.76	0.69
Fragment	0	0	32	1	2	1	2	0	0	0	38	0.84	0.73
Interphase	0	1	10	1,163	0	111	99	0	7	112	1,503	0.77	0.98
Metaphase	7	4	0	0	26	0	0	6	0	0	43	0.60	0.63
Multi-nuclear	1	1	0	9	1	100	0	3	0	10	125	0.80	0.46
Noncell	0	0	2	2	0	0	8	0	1	0	13	0.62	0.07
Prometaphase	12	0	0	0	8	1	0	28	7	0	56	0.50	0.62
Prophase	1	0	0	0	0	0	0	1	15	4	21	0.71	0.36
Telophase	0	11	0	8	0	3	1	1	9	95	128	0.74	0.42

Double thymidine block and release
	Classifier output
True class	Ana	Apop	Cyto	Inter	Meta	Multi-nuc	Noncell	Prom	Pro	Telo	#	TPR	PR
Anaphase	24	0	5	0	0	1	0	7	0	0	37	0.65	0.63
Apoptotic/Necrotic	1	29	0	0	0	1	1	0	0	0	32	0.91	0.91
Cytokinesis	5	0	50	0	0	2	0	0	0	13	70	0.71	0.77
Interphase	0	1	0	415	0	12	2	0	36	37	503	0.83	0.98
Metaphase	2	0	0	0	42	0	0	2	0	0	46	0.91	0.89
Multi-nuclear	1	1	0	2	0	54	0	0	3	0	61	0.89	0.74
Noncell	0	1	0	0	0	0	39	0	0	0	40	0.98	0.91
Prometaphase	5	0	0	0	5	0	1	71	2	0	84	0.85	0.82
Prophase	0	0	0	4	0	2	0	7	38	0	51	0.75	0.48
Telophase	0	0	10	4	0	1	0	0	1	53	69	0.77	0.51

Numbers in confusion matrices show the number of cells counted as a particular class by the classifier. # represents the total number of cells hand-scored as belonging to that class. Specificity (TPR) and precision (PR) are represented as ratios.