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. 2012 Mar 1;109(13):E757–E764. doi: 10.1073/pnas.1119414109

Fig. 3.

Fig. 3.

SgrS binds sopD in the proximal coding sequence. (A) Western and Northern blot analyses of Salmonella harboring plasmid pPL-SgrS or mutant plasmid pPL-SgrS* in combination with either WT sopD::gfp or mutant sopD*::gfp fusion plasmids. Protein and RNA samples were collected at OD600 of 2.0, and SopD::GFP protein levels were determined by Western blot analysis. SgrS and SgrS* levels were detected by Northern blot using a gene-specific oligonucleotide. (B) Graphical presentation of the SgrS–sopD interaction. Numbering for sopD relative to the start-codon AUG (A is +1) and SgrS relative to +1 of the transcription start site. The sopD coding region and SgrS are shown in capital letters. Vertical arrows denote nucleotides introduced in sopD::gfp and SgrS, respectively. (C) Graphical presentation of the SgrS–ptsG interaction for comparison. The gray bars indicate SgrS nucleotides shared in the interactions sopD and ptsG.