Fig. 6.

Systematic analysis of the SgrS–sopD/sopD2 interaction. (A) Graphical presentation of the SgrS-sopD/sopD2 duplexes and SgrS mutants used in B. Nucleotides exchanged in the SgrS mutants have been marked in gray. (B) Quantitative Western blot analysis of sopD::gfp and sopD2::gfp reporters combined with a control vector, pP_L-SgrS, pP_L-SgrS^A, pP_L-SgrS^C, or pP_L-SgrS^ΔA. Salmonella (ΔsgrS) double transformants were grown in rich media to OD₆₀₀ of 2.0, and total protein samples were subjected to Western blot analysis of GFP protein. Data are shown as fold repression relative to the control samples (average of three independent experiments). All SgrS variants are expressed equally (SI Appendix, Fig. S1).