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. 2012 Mar 12;109(13):4834-4839. doi: 10.1073/pnas.1114356109

Fig. 4.

Fig. 4.

FG-repeat nucleoporins are crosslinked through O-GlcNDAz residues. (A) HeLa cells transiently expressing AGX1(F383G) were cultured with Ac3GlcNDAz-1-P(Ac-SATE)2 (added 26 and/or 50 h after transfection). Cells were irradiated with 365 nm light, then lysed. Lysates were analyzed by immunoblot using a rabbit anti-NUP153 antibody. (B) HeLa cells stably expressing AGX1(F383G) were cultured with Ac3GlcNDAz-1-P(Ac-SATE)2. Irradiated and nonirradiated cells were lysed and lysates were immunoprecipitated with mAb414. Immunoprecipitates were separated by SDS-PAGE and visualized by silver staining. Indicated regions of the UV-irradiated sample were analyzed by mass spectrometry.