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. 2012 Mar 12;109(13):4881-4885. doi: 10.1073/pnas.1118452109

Fig. 2.

Fig. 2.

The rupture force distribution during early initiation steps. Each distributions fitted by Gaussian function have peak rupture force displayed above fitting curve. (A) 30S∶mRNA. (B) 30S∶tRNAfMet∶mRNA. (C) 30S∶IF2(GTP)∶fMet-tRNAfMet∶mRNA. (D) 70S∶fMet-tRNAfMet∶mRNA. (D) 70S∶IF2(GTP)∶fMet-tRNAfMet∶mRNA complexes. In the absence of IF2 50S subunit joining does not affect initiation complex stability. The presence of IF2 increases stability of the initiation complex by 5 pN upon large subunit joining. The arrow and bold arrow indicate no change and larger change in rupture force respectively.