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Cell-type-specific esterase-mediated unmasking in dissociated hippocampal neuron–astrocyte coculture. Neuron–astrocyte coculture was transfected with PLE–IRES–NLS-mCherry driven by the neuron-specific hSYN1 promoter and incubated with substrate 6 (10 μM) or substrate 1 (10 μM) for 30 min. (A) Selective unmasking observed when incubated with substrate 6. (B) Unselective unmasking observed when incubated with substrate 1. (Scale bars: 10 μm.)
