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. 2012 Mar 12;109(13):4886–4891. doi: 10.1073/pnas.1202120109

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Fig. 3. — Increased Pak activation in Ctnna1 cKO epidermis promotes Erk/MAPK phosphorylation. (A–E) Quantitative immunoblots of Pak1/2, Mek, and Erk1/2 phosphorylation levels from epidermal lysates from control and Ctnna1 cKO E18.5 embryos (A and B) and from their cultured keratinocytes (C and D). (E) Immunoblots of Mek phosphorylation upon in utero lentiviral transduction of Pak1 or scrambled shRNAs. (F) Quantifications of Pak1 knockdown efficiency. (G) Quantifications of relative Mek activity (phosphorylation) upon Pak1 knockdown.