Fig. 4.

Loss of junctional IGFR in Ctnna1-null keratinocytes. (A and B). Keratinocytes from WT and Ctnna1 cKO embryos were cultured in either low- or high-calcium medium and immunolabeled for IGFR, Paxillin, and/or E-cadherin as indicated. (C and D) Representative IFM images and quantifications to determine relative phospho-IGFR and IGFR levels in WT and Ctnna1-null keratinocytes in low and high calcium. (E) Planar views of WT and Ctnna1 cKO epidermis after immunolabeling E18.5 embryos for IGFR and E-cadherin. [Scale bars (A and B): 50 μm; (C and E): 20 μm.]