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. Author manuscript; available in PMC: 2012 Apr 11.
Published in final edited form as: Science. 2012 Mar 30;335(6076):1638–1643. doi: 10.1126/science.1215135

Figure 4.

Figure 4

Depletion of mTOR and mLST8 uncouples longevity from decreased glucose tolerance. A, B) Kaplan-Meier plots showing lifespans of female (A) and male (B) mice heterozygous for components of the mTOR signaling pathway. C, D) Lifespans of female (C) and male (D) mtor+/- mlst8+/- mice. Wild-type curves are repeated for comparison. E) Quantification of phosphorylated proteins in female wild-type and mtor+/- mlst8+/- livers after an overnight fast and 45 minutes of refeeding (n = 13 wild-type vs. 13 mtor+/- mlst8+/- female mice, * = P < 0.03). F) Q-PCR of mRNA levels for PEPCK and G6Pase in the livers of young female wild type and mtor+/- mlst8+/- mice (* = P < 0.03). G, H) Immunoprecipitation of mTOR complexes reveals preferential loss of Raptor association in mtor+/- mlst8+/- female mice (* = P < 0.02). All bars indicate mean and SEM.