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. Author manuscript; available in PMC: 2012 Apr 11.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Feb 5;30(3):436–441. doi: 10.1161/ATVBAHA.109.202135

Figure 5.

Figure 5

Mn-SOD inhibited oxLDL-induced apoptosis. HAEC were infected with human Mn-SOD adenoviral vector (Ad-Mn-SOD) or Ad-control at multiplicity of infection of 15 for 48 hours. A, Cell lysate was used to verify the efficiency of Ad-Mn-SOD on the Mn-SOD protein level. The blots were representative of 2 independent experiments with similar results. B, Effects of Ad-Mn-SOD on cell apoptosis. Cells were treated with 100 µg/mL of oxLDL for 24 hours (white block, no oxLDL; black block, with oxLDL). The cell lysates were collected and the caspase-3 activities were measured by enzyme-linked immunosorbent assay kit. These data represented the means from triplicates of 2 independent experiments. *P<0.05 vs in the absence of oxLDL and in the presence of Ad-control (lane 1). †P<0.05 vs in the presence of oxLDL and in the presence of Ad-control (lane 2).