Figure 5.

¹⁹F NMR experiments on HhRz-FU7 at higher Mg²⁺ concentrations. Plot of the chemical shift (ppm) of the FU7 ¹⁹F resonance as a function of added Mg²⁺ concentration (in the range 0 to 50 mM) in 10 mM Tris⋅HCl (pH 7.5), 10 mM NaCl. The line is the fit to the two-state model by using the data obtained at Mg²⁺ concentrations >1 mM.