Skip to main content
. 2011 Jul 1;2(3):90–99. doi: 10.4161/adna.18739

Figure 6.

Figure 6.

Downregulation of luciferase in p53R cells by a single stranded antisense siRNA (asRNA) delivered by carrier CPP-PNAs. Two octaarginine conjugated carrier CPP-PNAs (R8-cPNA, (PNA3164) and Deca-R8-cPNA (PNA3165)) with a complementary sequence to the asRNA and one PNA with non-related (NR) sequence but with analogous modifications (Deca-R8-PNA(NR), PNA2961)) as a control, were tested. The asRNA was hybridized to the carrier CPP-PNA at the molar ratio 1:1 and transfected to the p53R cells at the indicated concentrations in the absence or presence of 100 μM chloroquine (CQ). After 48 h transfection, luciferase activity was measured and is shown as relative luciferase activity (%) after normalization by protein concentration (non-siRNA treated sample set as 100%). Each data point represents the mean ± SD of three independent experiments.