Figure 4. PCA of nuclei in aging disorders. Principal component analysis was performed on control and disease groups of each disease. The panels on the left show the average nuclear shape of both the disease and control groups (red box) and the first 8 modes of PCA for each group. The x-axis shows image variations from the average shape of the nuclei. The graphs on the right show the variance of the control and disease group from the average shape (x-axis) per mode (y-axis). (A) In XFE nuclei, the most significant deformation modes were size and elongation, and the disease group had greater variation; (B) HGPS nuclei showed higher variation and deformation modes of size and elongation, and modes with bumps; (C) WS control and disease groups has similar variations in all modes.