Table 1. Primer sequences for PCR amplification of PAX6.
| Primer name | Primer sequence | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|
| PAX6-Exon4-f |
tgtaggggaaacaga |
55 |
491 |
| PAX6-Exon4-r |
atcgagaagagccaagcaaa |
|
|
| PAX6-Exon5-f |
ctggtggtcctgttgtcctt |
55 |
446 |
| PAX6-Exon5-r |
atgaagagagggcgttgaga |
|
|
| PAX6-Exon6-f |
gggctacaaatgtaattttaagaa |
55 |
509 |
| PAX6-Exon6-r |
agagagggtgggaggaggta |
|
|
| PAX6-Exon7-f |
gagctgagatgggtgactg |
55 |
300 |
| PAX6-Exon7-r |
gagagtaggggacaggcaaa |
|
|
| PAX6-Exon8-f |
tcaggtaactaacatcgca |
55 |
719 |
| PAX6-Exon8-r |
gttgactgtacttggaagaa |
|
|
| PAX6-Exon9-f |
aggtgggaaccagtttgatg |
55 |
311 |
| PAX6-Exon9-r |
catggcagcagagcatttag |
|
|
| PAX6-Exon10-f |
gctaaatgctctgctgccat |
55 |
329 |
| PAX6-Exon10-r |
agagtgagagtcagagcccg |
|
|
| PAX6-Exon11-f |
ccgggctctgactctcact |
55 |
221 |
| PAX6-Exon11-r |
gccactcctcacttctctgg |
|
|
| PAX6-Exon12-f |
gaggcttgatacataggc |
55 |
452 |
| PAX6-Exon12-r |
ccataagaccaggagatt |
|
|
| PAX6-Exon13-f |
tccatgtctgtttctcaaagg |
55 |
220 |
| PAX6-Exon13-r | tcaactgttgtgtccccatag |