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. 2012 Apr 11;7(4):e35117. doi: 10.1371/journal.pone.0035117

Figure 2. Identification of T-DNA flanking sequence in 54 A. thaliana lines by genome walking.

Figure 2

PCR amplification of sequences flanking the left border of T-DNA inserts (A) NsiI digested DNA. (B) NdeI digested DNA. The first lane for each row is 1 kb plus ladder (Invitrogen). (C) Example of T-DNA flanking sequence obtained from transgenic line p416 from the A. thaliana SK population. In this example, T-DNA was inserted into the TTG1 gene. The T-DNA footprint is highlighted in bold and the GW-adapter sequence is underlined.