Table 2. Fragment distribution frequency, methylation sensitivity and vector representation of 29 restriction enzymes with high numbers of fragments within a 100–3000 bp range in Arabidopsis.

Restriction enzyme	Fragments (%)			Methylation sensitive	Presence within vector pSKI015	Cohesive or blunt end
	<100 bp	0.1–3 kb	>3 kb
AluI	37.84	62.12	0.03	-	Y	B
AseI	15.84	71.51	12.66	-	Y	C
BfaI	23.54	76.27	0.19	-	Y	C
BglII	5.84	61.79	32.37	-	Y	C
BspHI	5.02	63.35	31.64	Dam	Y	C
BstUI	12.37	63.88	23.74	CG	Y	B
ChaI	34.83	65.13	0.03	?	Y	C
DpnI	34.83	65.13	0.03	Dam	Y	B
DraI	22.53	71.49	5.98	-	Y	B
FatI	33.69	66.30	0.01	-	Y	C
GlaI	9.04	67.04	23.91	-*	Y	B
HaeIII	17.07	76.75	6.17	-	Y	B
HhaI	9.04	67.04	23.91	CG	Y	C
HindIII	8.29	72.72	18.99	-	Y	C
HinP1I	9.04	67.04	23.91	-	Y	C
HpaII	25.06	69.04	5.90	CG	-	B
MboI	34.83	65.14	0.03	Dam, CG	Y	C
MseI	61.55	38.45	0.00	-	-	B
NdeI	4.99	59.09	35.93	-	-	C
NlaIII	33.69	66.30	0.01	-	Y	C
NsiI	5.76	63.88	30.37	-	-	C
PabI	20.39	79.13	0.48	?	Y	C
PciI	5.47	64.44	30.08	-	Y	C
PsiI	13.35	74.35	11.69	-	Y	B
RsaI	20.39	79.13	0.48	CG	Y	B
SelI	12.37	63.88	23.74	CG	-	C
SspI	17.21	75.12	7.67	-	Y	B
TaiI	22.73	76.47	0.80	CG	Y	C
TaqI	28.95	70.74	0.31	Dam	Y	C

^* GlaI is a methylation dependent endonuclease which only cleaves DNA when 5-methylcytosine or 5-hydroxymethylcytosine lies within its recognition sequence [34].

? information not available; Y, yes; B, blunt; C, cohesive.