Table 3. Fragment distribution frequency for in silico NsiI-digested fragments ≥3000 bp after in silico digestion with second restriction enzyme.

Secondary Restriction enzyme	Fragments (%)
	<100 bp	100–3000 bp	>3000 bp
BfaI	24.78	75.11	0.11
ChaI	36.32	63.66	0.02
NdeI	6.67	72.89	20.45
PciI	6.59	76.59	16.82
SelI	13.52	74.30	12.18
TaiI	23.79	75.72	0.49