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. 2012 Apr 11;7(4):e35115. doi: 10.1371/journal.pone.0035115

Figure 2. Proinflammatory stimuli enhance astrocyte PGRN Expression.

Figure 2

Astrocyte cultures were treated with IFNγ ± IL-1, poly IC, IL-4, IL-13 or medium alone (control) and PGRN expression was examined by ELISA and Q-PCR. (A) Secreted PGRN levels in control microglia and astrocyte cultures determined by ELISA (24 h) show that microglia produce larger amounts of PGRN than astrocytes. Each symbol represents a different case. (B) Representative ELISA data from cytokine-stimulated astrocyte cultures (24 h stimulation). (C, D) Pooled normalized Q-PCR data for PGRN and TNFα from astrocyte cultures (6 h stimulation, n = 5) are shown. Data are mean ± SD. * p<0.05, ** p<0.01, *** p<0.001. The results show that proinflammatory stimuli induce astrocyte PGRN production.